Dynamic Biosensors helix cyto Normalization Solution
spésifikasi
- Ngaran produk: heliX cyto Normalization Solution (Red Dye)
- Nomer pamesenan: NOR-R2
- For scIC measurements in the red channel
- Pikeun pamakéan panalungtikan wungkul
- Limited shelf life – please check expiry date on label
Fitur konci
- Pikeun normalisasi sinyal fluoresensi dina Spot 1 sareng Spot 2 tina chip heliXcyto
- Enables correct real-time referencing of the red fluorescent signals during scIC measurements
- Compatible with all heliXcyto chips
- The Normalization solution (red dye) contains a hydrophilic red dye with a single negative net charge.
Panjelasan Produk
- Nomer pamesenan: NOR-R2
meja 1. Eusi jeung Émbaran Panyimpenan
| Bahan | Cap | Concentr ation | Jumlah | Panyimpenan |
| Normalization solution-R2 | Beureum | 10 µM | 6 x 100 µl | -20°C |
- Pikeun pamakéan panalungtikan wungkul.
- This product has a limited shelf life; please see the expiry date on the label.
- To avoid many freeze-thaw cycles, please aliquot the solution.
Persiapan
- Use this red dye normalization solution for scIC measurements in the red channel (analyte label-dependent).
- Dilute the 10 µM normalization stock solution to a working concentration with running buffer.
- The concentration of the normalization solution should approximately correspond to the fluorophore concentration in the highest analyte concentration to be measured. This can be calculated usingthe following equation:
Concentration of the normalization solution in the desired color :
Konsentrasi pewarna dina larutan analit anu dilabélan
Highest concentration of the analyte that should be measured
Degree of labeling (ratio of dye to analyte)
Solusi anu éncér tiasa disimpen dina suhu 2-8 ° C dugi ka 7 dinten.
Catetan Aplikasi
In the scIC measurement, the fluorescent signal of the normalization solution should be in a similar range to the highest signal coming from the bound analyte (raw data). The absolute fluorescent signal is dependent on normalization solution concentration and the excitation power applied in the measurement. The excitation power has to be selected based on the following parameters:
- Fluorophore concentration inthe analyte solution
Konsentrasi fluorofor gumantung kana konsentrasi analit anu dianggo dina pangukuran sareng darajat panyiri analit. Pikeun DOL anu luhur sareng konsentrasi analit anu luhur, ngirangan kakuatan éksitasi tiasa diperyogikeun. - Sinyal beungkeutan diperkirakeun
Highly expressed targets on a cell can bind more molecules of the labeled analyte. In case of highly overexpressed targets, a strong binding signal can be expected. To avoid the shutter closing, lowering the excitation power might be considered. - Jenis chip
Jenis chip béda boga backgrounds fluoresensi varying. Langkung ageung sarap sareng langkung seueur sarap dina chip, langkung luhur sinyal latar. Ku alatan éta, chip L5 bisa merlukeun kakuatan éksitasi leuwih handap tina dilarapkeun ka chip M5.
For a starting point of excitation power and normSolution on concentration to be used in an scIC experiment, please refer to Table 2.
Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power suitable for an 
chip M5
| Conc. pewarna analit = analit conc x DOL | kakuatan éksitasi | Solusi Normalisasi Konsentrasi | Solusi Normalisasi Éncér |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 µM | 0.05 | 1 µM | 1:10 |
| 2.5 µM | 0.02 | 2.5 µM | 1:4 |
Catetan: This table is for your guidance. However, the final signal recorded in the heliXcyto depends on many factors. Thus, some optimization will be required for each system.
Kontak
Dinamis Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Germany
Bruker ilmiah LLC
40 Manning Jalan, Manning Park, Billerica, MA 01821 AS
- Émbaran pesenan
- Bantosan Téknis
www.dynamic-biosensors.com
Instrumén sareng chip direkayasa sareng diproduksi di Jerman. ©2025 Dynamic Biosensors GmbH Pikeun Pamakéan Panaliti Ngan. Henteu kanggo dianggo dina prosedur diagnostik klinis.
FAQs
What is the shelf life of the heliX cyto Normalization Solution?
produk ngabogaan umur rak kawates. Mangga parios tanggal kadaluwarsa dina labél kanggo inpormasi khusus.
How should I store the NOR-R2 v1.0 product?
The product should be stored according to the storage information provided in Table 1 of the user manual.
Can I use this product for clinical purposes?
No, this product is for research use only.
Dokumén / Sumberdaya
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Dynamic Biosensors helix cyto Normalization Solution [pdf] Manual pamaké helix cyto Normalization Solution, helix cyto, Normalization Solution, Solution |